cd3 apc h7 sk 7 cd16 pe cy7 b73 1  (Miltenyi Biotec)

Journal: Journal of Acquired Immune Deficiency Syndromes (1999)

Article Title: HIV-1 Elite Controllers Are Characterized by Elevated Levels of CD69-Expressing Natural Killer Cells

doi: 10.1097/QAI.0000000000003518

Figure Lengend Snippet: A, The percentage of total NK cells, CD56 bright CD16 - , CD56 dim CD16 + , and CD56 neg CD16+ NK cell subsets in people without HIV-1 (PWOH), PLWH on ART (ART), ECs, and VPs. Each symbol represents a study participant. The horizontal lines represent the medians, and the error bars represent the 25th and 75th percentiles. Significant P values are shown ( P < 0.05). B, The distribution of CD56 bright CD16 - , CD56 dim CD16 + , and CD56 neg CD16+ NK cell subsets in PWOH, ART, EC, and VPs. Areas represent the median percentages.

Article Snippet: Approximately 1 million thawed peripheral blood mononuclear cells (PBMCs) were immunophenotyped using CD3 PE-CF594 (UCHT1), CD19 BV711 (HIB19), CD14 PerCP-Cy5.5 (M5E2), CD56 Alexa Fluor 700 (B159), CD16 APC-Cy7 (3G8), CD69 FITC (FN50), PD-1 BV421 (EH12.1), CD57 BV605 (QA17A04) (all (Becton, Dickinson and Company) BD Biosciences, San Jose, USA), and CD38 PE-Cy7 (HIT2) (BioLegend, San Diego, USA) in the presence of BD Horizon Brilliant Stain Buffer (BD Biosciences).

Techniques:

Journal: Journal of Acquired Immune Deficiency Syndromes (1999)

Article Title: HIV-1 Elite Controllers Are Characterized by Elevated Levels of CD69-Expressing Natural Killer Cells

doi: 10.1097/QAI.0000000000003518

Figure Lengend Snippet: A, The percentage of CD107a + total NK cells, CD56 bright CD16 - , CD56 dim CD16 + , and CD56 neg CD16 + NK cell subsets in people without HIV-1 (PWOH), PLWH on ART (ART), ECs, and VPs. Correlations between the percentage of CD107a + total NK cells and viral load and CD4 + T-cell counts in PLWH combined. B, Correlations between the percentage of CD107a + total NK cells and CD4 + T-cell counts in PLWH on ART and EC. C, The percentage of IFN-γ + total NK cells, CD56 bright CD16 - , CD56 dim CD16 + , and CD56 neg CD16 + NK cell subsets in PWOH, ART, EC, and VPs. Correlations between the percentage of IFN-γ + total NK cells and viral load and CD4 + T-cell counts in PLWH combined. The horizontal lines represent the medians, and the error bars represent the 25th and 75th percentiles. Lines are calculated using linear regression. Each symbol represents a study participant. Significant P values ( P < 0.05; solid line), trends (0.05 < P < 0.08; dotted line), nonsignificant ( P > 0.05; no line), and Spearman rho (r) values are shown.

Article Snippet: Approximately 1 million thawed peripheral blood mononuclear cells (PBMCs) were immunophenotyped using CD3 PE-CF594 (UCHT1), CD19 BV711 (HIB19), CD14 PerCP-Cy5.5 (M5E2), CD56 Alexa Fluor 700 (B159), CD16 APC-Cy7 (3G8), CD69 FITC (FN50), PD-1 BV421 (EH12.1), CD57 BV605 (QA17A04) (all (Becton, Dickinson and Company) BD Biosciences, San Jose, USA), and CD38 PE-Cy7 (HIT2) (BioLegend, San Diego, USA) in the presence of BD Horizon Brilliant Stain Buffer (BD Biosciences).

Techniques:

Journal: Journal of Acquired Immune Deficiency Syndromes (1999)

Article Title: HIV-1 Elite Controllers Are Characterized by Elevated Levels of CD69-Expressing Natural Killer Cells

doi: 10.1097/QAI.0000000000003518

Figure Lengend Snippet: A, Glut1 + and (B) CD98 + expression, measured as median fluorescence intensity (MFI), on total NK cells, CD56 bright CD16 - , CD56 dim CD16 + , and CD56 neg CD16 + NK cell subsets in people without HIV-1 (PWOH), PLWH on ART (ART), ECs, and VPs. The horizontal lines represent the medians, and the error bars represent the 25th and 75th percentiles. Correlations between the expression of (A) Glut1 + and (B) CD98 + NK cells and viral load, and CD4 + T cells in PLWH combined. Lines are calculated using linear regression. Each symbol represents a study participant. Significant P values ( P < 0.05; solid line), trends (0.05 < P < 0.08; dotted line), nonsignificant ( P > 0.05; no line), and Spearman rho (r) values are shown.

Article Snippet: Approximately 1 million thawed peripheral blood mononuclear cells (PBMCs) were immunophenotyped using CD3 PE-CF594 (UCHT1), CD19 BV711 (HIB19), CD14 PerCP-Cy5.5 (M5E2), CD56 Alexa Fluor 700 (B159), CD16 APC-Cy7 (3G8), CD69 FITC (FN50), PD-1 BV421 (EH12.1), CD57 BV605 (QA17A04) (all (Becton, Dickinson and Company) BD Biosciences, San Jose, USA), and CD38 PE-Cy7 (HIT2) (BioLegend, San Diego, USA) in the presence of BD Horizon Brilliant Stain Buffer (BD Biosciences).

Techniques: Expressing, Fluorescence

Journal: Cell reports

Article Title: Beta-spike-containing boosters induce robust and functional antibody responses to SARS-CoV-2 in macaques primed with distinct vaccines

doi: 10.1016/j.celrep.2023.113292

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-human CD16 (clone 3G8), APC-Cy7 , BD Biosciences , AB_396864.

Techniques: Recombinant, Virus, Software, Labeling, Luminex

Journal: Digestive Diseases and Sciences

Article Title: Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer

doi: 10.1007/s10620-024-08536-0

Figure Lengend Snippet: Downregulation of peripheral blood NK cell-activating receptors NKG2D and NKp30 and cytokine factors IFN-γ and TNF-α in gastric cancer. A – C NK cell gating strategy. D and E Flow cytometry analysis comparing the expression of NK cell subsets CD3−/CD56+ and CD3−/CD16+ in GC and HD peripheral blood. F – H Flow cytometry analysis comparing the expression of NK cell receptors NKG2D, NKp30, and NKp46 in GC and HD peripheral blood. I and J ELISA detection of cytokine IFN-γ and TNF-α expression in GC (n = 16) and HD (n = 16) plasma. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant

Article Snippet: Single-cell suspensions were stained using the following antibodies: anti-human CD3 PE (BioGems, USA), anti-human CD16 PE-Cy7 (BioGems), anti-human CD56 APC (BioGems), anti-human NKG2D (CD314) PE (BioGems), anti-human NKp30(CD337) PE-Cy7 (BD, USA), anti-human NKp46(CD335) APC (BD), and PD-L1 PE (CST, USA).

Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Digestive Diseases and Sciences

Article Title: Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer

doi: 10.1007/s10620-024-08536-0

Figure Lengend Snippet: Correlation of NK cell phenotype and activation receptor gene expression with prognosis in gastric cancer. Kaplan–Meier survival analysis revealed the following associations with poor prognosis in gastric cancer patients: low CD56 expression ( A ), low CD16 expression ( B ), low NKG2D expression ( C ), and low NKp46 expression ( E ). However, no correlation between NKp30 expression and prognosis was observed ( D )

Article Snippet: Single-cell suspensions were stained using the following antibodies: anti-human CD3 PE (BioGems, USA), anti-human CD16 PE-Cy7 (BioGems), anti-human CD56 APC (BioGems), anti-human NKG2D (CD314) PE (BioGems), anti-human NKp30(CD337) PE-Cy7 (BD, USA), anti-human NKp46(CD335) APC (BD), and PD-L1 PE (CST, USA).

Techniques: Activation Assay, Gene Expression, Expressing

Journal: Digestive Diseases and Sciences

Article Title: Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer

doi: 10.1007/s10620-024-08536-0

Figure Lengend Snippet: Correlation of plasma exosomal miR-552-5p with NK cell phenotypes and activated receptors in gastric cancer. A and B Negative correlation between plasma exosomal miR-552-5p expression and the distribution of CD3−/CD56+ and CD3−/CD16+ subpopulations in peripheral blood NK cells of gastric cancer patients. C – E Negative correlation between plasma exosomal miR-552-5p expression and the expression of peripheral blood NK cell-activating receptors NKG2D, NKp30, and NKp46 in gastric cancer

Article Snippet: Single-cell suspensions were stained using the following antibodies: anti-human CD3 PE (BioGems, USA), anti-human CD16 PE-Cy7 (BioGems), anti-human CD56 APC (BioGems), anti-human NKG2D (CD314) PE (BioGems), anti-human NKp30(CD337) PE-Cy7 (BD, USA), anti-human NKp46(CD335) APC (BD), and PD-L1 PE (CST, USA).

Techniques: Clinical Proteomics, Expressing